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Clinical

Expression data from human macrophages -GSE16385

Purpose

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Hypothesis

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Experimental Design

Human CD14 positive monocytes were purified from healthy volunteers’ blood and cultured in vitro for 4, 12, 24, 72 hours. While culturing, macrophages were activated alternatively with interleukin-4 (IL-4 100 ng/ml) or classically with interferon-gamma (IFNg 100 ng/ml)+tumor necrosis factor (TNF 50 ng/ml) or left without activation. Simultaneously, macrophages were also treated with vehicle (DMSO:ethanol) or 1mM synthetic PPARg agonist, Rosiglitazone. We used Affymetrix microarrays (U133Plus 2.0) to analyze activation and PPARg-induced gene expression changes.

Experimental Variables

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Controls

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Methods

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Additional Information
  • Szanto A, Balint BL, Nagy ZS, Barta E et al. STAT6 transcription factor is a facilitator of the nuclear receptor PPAR?-regulated gene expression in macrophages and dendritic cells. Immunity 2010 Nov 24;33(5):699-712. PMID: 21093321
  • Microarray
    Affymetrix HG-U133_Plus_2
    38 Samples Loaded: 38
    Human (Homo sapiens)
    Monocytes
    Healthy
    Sample Set Spreadsheet
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    Samples Preview
    Sample ID !Sample_title donor treatment time tissue
    GSM411183 1 Monocyte 1 None 0h monocyte
    GSM411184 1 Control macrophage, 4h 1 Control 4h macrophage
    GSM411185 1 Control macrophage+Rosigl., 4h 1 Rosiglitazone 4h macrophage
    GSM411186 1 IL-4 macrophage, 4h 1 Control 4h macrophage
    GSM411187 1 IL-4 macrophage+Rosigl., 4h 1 Rosiglitazone 4h macrophage
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    Sample ID !Sample Title Donor Treatment Time Tissue Activation
    GSM411183
    1 Monocyte
    1
    None
    0h
    monocyte
    None
    GSM411184
    1 Control macrophage, 4h
    1
    Control
    4h
    macrophage
    Control
    GSM411185
    1 Control macrophage+Rosigl., 4h
    1
    Rosiglitazone
    4h
    macrophage
    Control
    GSM411186
    1 IL-4 macrophage, 4h
    1
    Control
    4h
    macrophage
    IL-4
    GSM411187
    1 IL-4 macrophage+Rosigl., 4h
    1
    Rosiglitazone
    4h
    macrophage
    IL-4
    GSM411188
    1 IFNg+TNF macrophage, 4h
    1
    Control
    4h
    macrophage
    IFNg+TNF
    GSM411189
    1 IFNg+TNF macrophage+Rosigl., 4h
    1
    Rosiglitazone
    4h
    macrophage
    IFNg+TNF
    GSM411190
    1 Control macrophage, 12h
    1
    Control
    12h
    macrophage
    Control
    GSM411191
    1 Control macrophage+Rosigl., 12h
    1
    Rosiglitazone
    12h
    macrophage
    Control
    GSM411192
    1 IL-4 macrophage, 12h
    1
    Control
    12h
    macrophage
    IL-4
    GSM411193
    1 IL-4 macrophage+Rosigl., 12h
    1
    Rosiglitazone
    12h
    macrophage
    IL-4
    GSM411194
    1 IFNg+TNF macrophage,12h
    1
    Control
    12h
    macrophage
    IFNg+TNF
    GSM411195
    1 IFNg+TNF macrophage+Rosigl., 12h
    1
    Rosiglitazone
    12h
    macrophage
    IFNg+TNF
    GSM411196
    2 Monocyte
    2
    None
    0h
    monocyte
    None
    GSM411197
    2 Control macrophage, 12h
    2
    Control
    12h
    macrophage
    Control
    GSM411198
    2 Control macrophage+Rosigl., 12h
    2
    Rosiglitazone
    12h
    macrophage
    Control
    GSM411199
    2 IL-4 macrophage, 12h
    2
    Control
    12h
    macrophage
    IL-4
    GSM411200
    2 IL-4 macrophage+Rosigl., 12h
    2
    Rosiglitazone
    12h
    macrophage
    IL-4
    GSM411201
    2 IFNg+TNF macrophage, 12h
    2
    Control
    12h
    macrophage
    IFNg+TNF
    GSM411202
    2 IFNg+TNF macrophage+Rosigl., 12h
    2
    Rosiglitazone
    12h
    macrophage
    IFNg+TNF
    GSM411203
    2 Control macrophage, 24h
    2
    Control
    24h
    macrophage
    Control
    GSM411204
    2 Control macrophage+Rosigl., 24h
    2
    Rosiglitazone
    24h
    macrophage
    Control
    GSM411205
    2 IL-4 macrophage, 24h
    2
    Control
    24h
    macrophage
    IL-4
    GSM411206
    2 IL-4 macrophage+Rosigl., 24h
    2
    Rosiglitazone
    24h
    macrophage
    IL-4
    GSM411207
    2 IFNg+TNF macrophage, 24h
    2
    Control
    24h
    macrophage
    IFNg+TNF
    GSM411208
    2 IFNg+TNF macrophage+Rosigl., 24h
    2
    Rosiglitazone
    24h
    macrophage
    IFNg+TNF
    GSM411209
    3 Control macrophage, 12h
    3
    Control
    12h
    macrophage
    Control
    GSM411210
    3 Control macrophage+Rosigl., 12h
    3
    Rosiglitazone
    12h
    macrophage
    Control
    GSM411211
    3 IL-4 macrophage, 12h
    3
    Control
    12h
    macrophage
    IL-4
    GSM411212
    3 IL-4 macrophage+Rosigl., 12h
    3
    Rosiglitazone
    12h
    macrophage
    IL-4
    GSM411213
    3 IFNg+TNF macrophage, 12h
    3
    Control
    12h
    macrophage
    IFNg+TNF
    GSM411214
    3 IFNg+TNF macrophage+Rosigl., 12h
    3
    Rosiglitazone
    12h
    macrophage
    IFNg+TNF
    GSM411215
    3 Control macrophage, 72h
    3
    Control
    72h
    macrophage
    Control
    GSM411216
    3 Control macrophage+Rosigl., 72h
    3
    Rosiglitazone
    72h
    macrophage
    Control
    GSM411217
    3 IL-4 macrophage, 72h
    3
    Control
    72h
    macrophage
    IL-4
    GSM411218
    3 IL-4 macrophage+Rosigl., 72h
    3
    Rosiglitazone
    72h
    macrophage
    IL-4
    GSM411219
    3 IFNg+TNF macrophage, 72h
    3
    Control
    72h
    macrophage
    IFNg+TNF
    GSM411220
    3 IFNg+TNF macrophage+Rosigl., 72h
    3
    Rosiglitazone
    72h
    macrophage
    IFNg+TNF

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