Effects of PMN-Ectosomes on human macrophages-GSE14419
In order to define the inhibitory activity of PMN-Ectosomes, we investigated the early gene expression profiles of resting and zymosan A-stimulated human monocyte-derived macrophages in the absence or presence of PMN-Ectosomes.
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We cultured monocytes isolated from fresh buffy coats of 4 healthy donors (2 males and 2 females between the ages of 20 to 45) for 7 days at 37°C. At day 7, macrophages were cultured for another 3 hours alone, in presence of PMN-Ectosomes, with zymosan A, and with zymosan A and PMN-Ectosomes.
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Sample ID | !Sample_title | donor | treatment | Grouping |
---|---|---|---|---|
GSM360139 | Don1_Macrophages alone | Don1 | Macrophages alone | Control |
GSM360140 | Don1_Macrophages+PMN-Ectosomes | Don1 | Macrophages+PMN-Ectosomes | PMN-Ecto |
GSM360141 | Don1_Macrophages+ZymosanA | Don1 | Macrophages+ZymosanA | Zym A |
GSM360142 | Don1_Macrophages+ZymosanA+PMN-Ectosomes | Don1 | Macrophages+ZymosanA+PMN-Ectosomes | Zym A + PMN-Ecto |
GSM360143 | Don2_Macrophages alone | Don2 | Macrophages alone | Control |
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Sample ID | !Sample Title | Donor | Treatment | Grouping |
---|---|---|---|---|
GSM360139 | Don1_Macrophages alone |
Don1 |
Macrophages alone |
Control |
GSM360140 | Don1_Macrophages+PMN-Ectosomes |
Don1 |
Macrophages+PMN-Ectosomes |
PMN-Ecto |
GSM360141 | Don1_Macrophages+ZymosanA |
Don1 |
Macrophages+ZymosanA |
Zym A |
GSM360142 | Don1_Macrophages+ZymosanA+PMN-Ectosomes |
Don1 |
Macrophages+ZymosanA+PMN-Ectosomes |
Zym A + PMN-Ecto |
GSM360143 | Don2_Macrophages alone |
Don2 |
Macrophages alone |
Control |
GSM360144 | Don2_Macrophages+PMN-Ectosomes |
Don2 |
Macrophages+PMN-Ectosomes |
PMN-Ecto |
GSM360145 | Don2_Macrophages+ZymosanA |
Don2 |
Macrophages+ZymosanA |
Zym A |
GSM360146 | Don2_Macrophages+ZymosanA+PMN-Ectosomes |
Don2 |
Macrophages+ZymosanA+PMN-Ectosomes |
Zym A + PMN-Ecto |
GSM360182 | Don3_Macrophages alone |
Don3 |
Macrophages alone |
Control |
GSM360183 | Don3_Macrophages+PMN-Ectosomes |
Don3 |
Macrophages+PMN-Ectosomes |
PMN-Ecto |
GSM360184 | Don3_Macrophages+ZymosanA |
Don3 |
Macrophages+ZymosanA |
Zym A |
GSM360185 | Don3_Macrophages+ZymosanA+PMN-Ectosomes |
Don3 |
Macrophages+ZymosanA+PMN-Ectosomes |
Zym A + PMN-Ecto |
GSM360186 | Don4_Macrophages alone |
Don4 |
Macrophages alone |
Control |
GSM360187 | Don4_Macrophages+PMN-Ectosomes |
Don4 |
Macrophages+PMN-Ectosomes |
PMN-Ecto |
GSM360188 | Don4_Macrophages+ZymosanA |
Don4 |
Macrophages+ZymosanA |
Zym A |
GSM360221 | Don4_Macrophages+ZymosanA+PMN-Ectosomes |
Don4 |
Macrophages+ZymosanA+PMN-Ectosomes |
Zym A + PMN-Ecto |
Name | ||
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