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Clinical

Effects of PMN-Ectosomes on human macrophages-GSE14419

Purpose

In order to define the inhibitory activity of PMN-Ectosomes, we investigated the early gene expression profiles of resting and zymosan A-stimulated human monocyte-derived macrophages in the absence or presence of PMN-Ectosomes.

Hypothesis

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Experimental Design

We cultured monocytes isolated from fresh buffy coats of 4 healthy donors (2 males and 2 females between the ages of 20 to 45) for 7 days at 37°C. At day 7, macrophages were cultured for another 3 hours alone, in presence of PMN-Ectosomes, with zymosan A, and with zymosan A and PMN-Ectosomes.

Experimental Variables

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Controls

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Methods

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Additional Information

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Microarray
Affymetrix HG-U133A
16 Samples Loaded: 16
Human (Homo sapiens)
Neutrophils , Macrophages
Healthy
Sample Set Spreadsheet
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Samples Preview
Sample ID !Sample_title donor treatment Grouping
GSM360139 Don1_Macrophages alone Don1 Macrophages alone Control
GSM360140 Don1_Macrophages+PMN-Ectosomes Don1 Macrophages+PMN-Ectosomes PMN-Ecto
GSM360141 Don1_Macrophages+ZymosanA Don1 Macrophages+ZymosanA Zym A
GSM360142 Don1_Macrophages+ZymosanA+PMN-Ectosomes Don1 Macrophages+ZymosanA+PMN-Ectosomes Zym A + PMN-Ecto
GSM360143 Don2_Macrophages alone Don2 Macrophages alone Control
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File
sampleset4000015_sampleannotations.csv
Sample Set Spreadsheet
Raw Signal
Raw Signal
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Samples Viewer / Editor

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Sample ID !Sample Title Donor Treatment Grouping
GSM360139
Don1_Macrophages alone
Don1
Macrophages alone
Control
GSM360140
Don1_Macrophages+PMN-Ectosomes
Don1
Macrophages+PMN-Ectosomes
PMN-Ecto
GSM360141
Don1_Macrophages+ZymosanA
Don1
Macrophages+ZymosanA
Zym A
GSM360142
Don1_Macrophages+ZymosanA+PMN-Ectosomes
Don1
Macrophages+ZymosanA+PMN-Ectosomes
Zym A + PMN-Ecto
GSM360143
Don2_Macrophages alone
Don2
Macrophages alone
Control
GSM360144
Don2_Macrophages+PMN-Ectosomes
Don2
Macrophages+PMN-Ectosomes
PMN-Ecto
GSM360145
Don2_Macrophages+ZymosanA
Don2
Macrophages+ZymosanA
Zym A
GSM360146
Don2_Macrophages+ZymosanA+PMN-Ectosomes
Don2
Macrophages+ZymosanA+PMN-Ectosomes
Zym A + PMN-Ecto
GSM360182
Don3_Macrophages alone
Don3
Macrophages alone
Control
GSM360183
Don3_Macrophages+PMN-Ectosomes
Don3
Macrophages+PMN-Ectosomes
PMN-Ecto
GSM360184
Don3_Macrophages+ZymosanA
Don3
Macrophages+ZymosanA
Zym A
GSM360185
Don3_Macrophages+ZymosanA+PMN-Ectosomes
Don3
Macrophages+ZymosanA+PMN-Ectosomes
Zym A + PMN-Ecto
GSM360186
Don4_Macrophages alone
Don4
Macrophages alone
Control
GSM360187
Don4_Macrophages+PMN-Ectosomes
Don4
Macrophages+PMN-Ectosomes
PMN-Ecto
GSM360188
Don4_Macrophages+ZymosanA
Don4
Macrophages+ZymosanA
Zym A
GSM360221
Don4_Macrophages+ZymosanA+PMN-Ectosomes
Don4
Macrophages+ZymosanA+PMN-Ectosomes
Zym A + PMN-Ecto

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