Sample Set Annotation Tool: Effects of PMN-Ectosomes on human macrophages-GSE14419

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

In order to define the inhibitory activity of PMN-Ectosomes, we investigated the early gene expression profiles of resting and zymosan A-stimulated human monocyte-derived macrophages in the absence or presence of PMN-Ectosomes.

Hypothesis

Experimental Design

We cultured monocytes isolated from fresh buffy coats of 4 healthy donors (2 males and 2 females between the ages of 20 to 45) for 7 days at 37°C. At day 7, macrophages were cultured for another 3 hours alone, in presence of PMN-Ectosomes, with zymosan A, and with zymosan A and PMN-Ectosomes.

Experimental Variables

Controls

Methods

Additional Information

Expected Samples:

16 Samples Loaded: 16

Species

Human (Homo sapiens)

Sample Source

Neutrophils , Macrophages

Disease

Healthy

Treatment Protocol

Growth Protocol

Extraction Protocol

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	!Sample_title	donor	treatment	Grouping
GSM360139	Don1_Macrophages alone	Don1	Macrophages alone	Control
GSM360140	Don1_Macrophages+PMN-Ectosomes	Don1	Macrophages+PMN-Ectosomes	PMN-Ecto
GSM360141	Don1_Macrophages+ZymosanA	Don1	Macrophages+ZymosanA	Zym A
GSM360142	Don1_Macrophages+ZymosanA+PMN-Ectosomes	Don1	Macrophages+ZymosanA+PMN-Ectosomes	Zym A + PMN-Ecto
GSM360143	Don2_Macrophages alone	Don2	Macrophages alone	Control

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File
sampleset4000015_sampleannotations.csv Sample Set Spreadsheet

Samples Viewer / Editor

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Sample ID	!Sample Title	Donor	Treatment	Grouping
GSM360139	Don1_Macrophages alone	Don1	Macrophages alone	Control
GSM360140	Don1_Macrophages+PMN-Ectosomes	Don1	Macrophages+PMN-Ectosomes	PMN-Ecto
GSM360141	Don1_Macrophages+ZymosanA	Don1	Macrophages+ZymosanA	Zym A
GSM360142	Don1_Macrophages+ZymosanA+PMN-Ectosomes	Don1	Macrophages+ZymosanA+PMN-Ectosomes	Zym A + PMN-Ecto
GSM360143	Don2_Macrophages alone	Don2	Macrophages alone	Control
GSM360144	Don2_Macrophages+PMN-Ectosomes	Don2	Macrophages+PMN-Ectosomes	PMN-Ecto
GSM360145	Don2_Macrophages+ZymosanA	Don2	Macrophages+ZymosanA	Zym A
GSM360146	Don2_Macrophages+ZymosanA+PMN-Ectosomes	Don2	Macrophages+ZymosanA+PMN-Ectosomes	Zym A + PMN-Ecto
GSM360182	Don3_Macrophages alone	Don3	Macrophages alone	Control
GSM360183	Don3_Macrophages+PMN-Ectosomes	Don3	Macrophages+PMN-Ectosomes	PMN-Ecto
GSM360184	Don3_Macrophages+ZymosanA	Don3	Macrophages+ZymosanA	Zym A
GSM360185	Don3_Macrophages+ZymosanA+PMN-Ectosomes	Don3	Macrophages+ZymosanA+PMN-Ectosomes	Zym A + PMN-Ecto
GSM360186	Don4_Macrophages alone	Don4	Macrophages alone	Control
GSM360187	Don4_Macrophages+PMN-Ectosomes	Don4	Macrophages+PMN-Ectosomes	PMN-Ecto
GSM360188	Don4_Macrophages+ZymosanA	Don4	Macrophages+ZymosanA	Zym A
GSM360221	Don4_Macrophages+ZymosanA+PMN-Ectosomes	Don4	Macrophages+ZymosanA+PMN-Ectosomes	Zym A + PMN-Ecto

Group Sets View in Gene Expression Browser

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