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Clinical

Interleukin-1- and Type I Interferon-Dependent Enhanced Immunogenicity of an NYVAC-HIV-1 Env-Gag-Pol-Nef Vaccine Vector with Dual Deletions of Type I and Type II Interferon-Binding Proteins-GSE65412

Purpose

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Hypothesis

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Experimental Design

Primary Human monocytes from 5 donors were incubated with each following vectors nyvacdb, nyvadb19r, nyvaccdb8rb19r_6h, untreated control and collected at 6h.

Experimental Variables

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Controls

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Methods

Primary monocytes

Additional Information
  • Delaloye J, Filali-Mouhim A, Cameron MJ, Haddad EK et al. Interleukin-1- and type I interferon-dependent enhanced immunogenicity of an NYVAC-HIV-1 Env-Gag-Pol-Nef vaccine vector with dual deletions of type I and type II interferon-binding proteins. J Virol 2015 Apr;89(7):3819-32. PMID: 25609807
  • Microarray
    Illumina HumanHT-12 v4
    20 Samples Loaded: 20
    Human (Homo sapiens)
    Monocytes
    Vaccination
    Sample Set Spreadsheet
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    Samples Preview
    Sample ID !Sample_title donor id timepoint cell type sample group
    GSM1595760 control Donor 1 [5727946083_B] donor 1 0hr primary human monocytes control
    GSM1595761 control Donor 2 [5826629001_J] donor 2 0hr primary human monocytes control
    GSM1595762 control Donor 3 [5826629022_E] donor 3 0hr primary human monocytes control
    GSM1595763 control Donor 4 [5826629089_F] donor 4 0hr primary human monocytes control
    GSM1595764 control Donor 5 [5826629031_C] donor 5 0hr primary human monocytes control
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    Log2 Transformed
    Raw Signal
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    Sample ID !Sample Title Donor id Timepoint Cell type Sample group Infected with
    GSM1595760
    control Donor 1 [5727946083_B]
    donor 1
    0hr
    primary human monocytes
    control
    none (control)
    GSM1595761
    control Donor 2 [5826629001_J]
    donor 2
    0hr
    primary human monocytes
    control
    none (control)
    GSM1595762
    control Donor 3 [5826629022_E]
    donor 3
    0hr
    primary human monocytes
    control
    none (control)
    GSM1595763
    control Donor 4 [5826629089_F]
    donor 4
    0hr
    primary human monocytes
    control
    none (control)
    GSM1595764
    control Donor 5 [5826629031_C]
    donor 5
    0hr
    primary human monocytes
    control
    none (control)
    GSM1595765
    nyvacc_6h Donor 1 [5727946083_E]
    donor 1
    6hrs
    primary human monocytes
    infected
    nyvacc for 1hr
    GSM1595766
    nyvacc_6h Donor 2 [5826629022_I]
    donor 2
    6hrs
    primary human monocytes
    infected
    nyvacc for 1hr
    GSM1595767
    nyvacc_6h Donor 3 [5727946083_C]
    donor 3
    6hrs
    primary human monocytes
    infected
    nyvacc for 1hr
    GSM1595768
    nyvacc_6h Donor 4 [5826629022_H]
    donor 4
    6hrs
    primary human monocytes
    infected
    nyvacc for 1hr
    GSM1595769
    nyvacc_6h Donor 5 [5826629029_C]
    donor 5
    6hrs
    primary human monocytes
    infected
    nyvacc for 1hr
    GSM1595770
    nyvaccdb19r_6h Donor 1 [5727946084_B]
    donor 1
    6hrs
    primary human monocytes
    infected
    nyvaccdb19r for 1hr
    GSM1595771
    nyvaccdb19r_6h Donor 2 [5727946083_I]
    donor 2
    6hrs
    primary human monocytes
    infected
    nyvaccdb19r for 1hr
    GSM1595772
    nyvaccdb19r_6h Donor 3 [5727946083_L]
    donor 3
    6hrs
    primary human monocytes
    infected
    nyvaccdb19r for 1hr
    GSM1595773
    nyvaccdb19r_6h Donor 4 [5826629001_C]
    donor 4
    6hrs
    primary human monocytes
    infected
    nyvaccdb19r for 1hr
    GSM1595774
    nyvaccdb19r_6h Donor 5 [5826629025_D]
    donor 5
    6hrs
    primary human monocytes
    infected
    nyvaccdb19r for 1hr
    GSM1595775
    nyvaccdb8rb19r_6h Donor 1 [5727946084_I]
    donor 1
    6hrs
    primary human monocytes
    infected
    nyvaccd8rb19r for 1hr
    GSM1595776
    nyvaccdb8rb19r_6h Donor 2 [5727946084_K]
    donor 2
    6hrs
    primary human monocytes
    infected
    nyvaccd8rb19r for 1hr
    GSM1595777
    nyvaccdb8rb19r_6h Donor 3 [5826629022_C]
    donor 3
    6hrs
    primary human monocytes
    infected
    nyvaccd8rb19r for 1hr
    GSM1595778
    nyvaccdb8rb19r_6h Donor 4 [5727946084_F]
    donor 4
    6hrs
    primary human monocytes
    infected
    nyvaccd8rb19r for 1hr
    GSM1595779
    nyvaccdb8rb19r_6h Donor 5 [5826629001_K]
    donor 5
    6hrs
    primary human monocytes
    infected
    nyvaccd8rb19r for 1hr

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