Sample Set Annotation Tool: Effect of vitamin D treatment on human monocytes-GSE56490

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

We used microarrays to investigate the global program of gene expression in human differentiating monocytes in the presence of the bio-active from of vitamin D.

Hypothesis

Experimental Design

Human CD14+ monocytes were isolated from PBMCs of healhty donors and cultured in the presence of 1,25(OH)2D3 (10-8M) or ethanol for 4h or 24h. Total RNA was then isolated and analyzed by microarray. Four monocytic preparations from different healthy donors were used.

Experimental Variables

Controls

Methods

Additional Information

Expected Samples:

16 Samples Loaded: 16

Species

Human (Homo sapiens)

Sample Source

Monocytes , Monocyte-derived macrophages (MDMs) , Macrophages

Disease

Healthy

Treatment Protocol

Growth Protocol

Extraction Protocol

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	!Sample_title	cell type	Grouping
GSM1362464	Differentiating monocytes at 4h-CTR rep1	PBMCs from buffy coats	Control 4 h
GSM1362465	Differentiating monocytes at 24h-CTR rep1	PBMCs from buffy coats	Control 24 h
GSM1362466	Differentiating monocytes at 4h-VD rep1	PBMCs from buffy coats	Vit D 4 h
GSM1362467	Differentiating monocytes at 24h-VD rep1	PBMCs from buffy coats	Vit D 24 h
GSM1362468	Differentiating monocytes at 4h-CTR rep2	PBMCs from buffy coats	Control 4 h

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File
sampleset4000005_sampleannotations.csv Sample Set Spreadsheet

Samples Viewer / Editor

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Sample ID	!Sample Title	Cell type	Grouping
GSM1362464	Differentiating monocytes at 4h-CTR rep1	PBMCs from buffy coats	Control 4 h
GSM1362465	Differentiating monocytes at 24h-CTR rep1	PBMCs from buffy coats	Control 24 h
GSM1362466	Differentiating monocytes at 4h-VD rep1	PBMCs from buffy coats	Vit D 4 h
GSM1362467	Differentiating monocytes at 24h-VD rep1	PBMCs from buffy coats	Vit D 24 h
GSM1362468	Differentiating monocytes at 4h-CTR rep2	PBMCs from buffy coats	Control 4 h
GSM1362469	Differentiating monocytes at 24h-CTR rep2	PBMCs from buffy coats	Control 24 h
GSM1362470	Differentiating monocytes at 4h-VD rep2	PBMCs from buffy coats	Vit D 4 h
GSM1362471	Differentiating monocytes at 24h-VD rep2	PBMCs from buffy coats	Vit D 24 h
GSM1362472	Differentiating monocytes at 4h-CTR rep3	PBMCs from buffy coats	Control 4 h
GSM1362473	Differentiating monocytes at 24h-CTR rep3	PBMCs from buffy coats	Control 24 h
GSM1362474	Differentiating monocytes at 4h-VD rep3	PBMCs from buffy coats	Vit D 4 h
GSM1362475	Differentiating monocytes at 24h-VD rep3	PBMCs from buffy coats	Vit D 24 h
GSM1362476	Differentiating monocytes at 4h-CTR rep4	PBMCs from buffy coats	Control 4 h
GSM1362477	Differentiating monocytes at 24h-CTR rep4	PBMCs from buffy coats	Control 24 h
GSM1362478	Differentiating monocytes at 4h-VD rep4	PBMCs from buffy coats	Vit D 4 h
GSM1362479	Differentiating monocytes at 24h-VD rep4	PBMCs from buffy coats	Vit D 24 h

Group Sets View in Gene Expression Browser

Name
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Module Analysis

Group Set
All Samples

SIDRA