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Clinical

Alarmins MRP8 and MRP14 induce stress-tolerance in phagocytes under sterile inflammatory conditions-GSE61477

Purpose

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Hypothesis

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Experimental Design

Human blood monocytes prestimulated with MRP8-MRP14 or LPS and afterwards activated with LPS were selected for RNA extraction and hybridization on Illumina microarrays.

Experimental Variables

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Controls

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Methods

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Additional Information
  • Austermann J, Friesenhagen J, Fassl SK, Petersen B et al. Alarmins MRP8 and MRP14 induce stress tolerance in phagocytes under sterile inflammatory conditions. Cell Rep 2014 Dec 24;9(6):2112-23. PMID: 25497086
  • Microarray
    Illumina HumanHT-12 v4
    12 Samples Loaded: 12
    Human (Homo sapiens)
    Monocytes
    Sterile Inflammation
    Sample Set Spreadsheet
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    Samples Preview
    Sample ID !Sample_title treatment cell type
    GSM1505864 LPS-con 1.7 24h LPS 25pg n 4h human blood monocyte
    GSM1505865 LPS-con 2.7 24h LPS 25pg n 4h human blood monocyte
    GSM1505866 LPS-con 3.7 24h LPS 25pg n 4h human blood monocyte
    GSM1505867 MRP8/14-con 1.8 24h MRP8-14 10µg n 4h human blood monocyte
    GSM1505868 MRP8/14-con 2.8 24h MRP8-14 10µg n 4h human blood monocyte
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    Log2 Transformed
    Raw Signal
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    Sample ID !Sample Title Treatment Cell type
    GSM1505864
    LPS-con 1.7
    24h LPS 25pg n 4h
    human blood monocyte
    GSM1505865
    LPS-con 2.7
    24h LPS 25pg n 4h
    human blood monocyte
    GSM1505866
    LPS-con 3.7
    24h LPS 25pg n 4h
    human blood monocyte
    GSM1505867
    MRP8/14-con 1.8
    24h MRP8-14 10µg n 4h
    human blood monocyte
    GSM1505868
    MRP8/14-con 2.8
    24h MRP8-14 10µg n 4h
    human blood monocyte
    GSM1505869
    MRP8/14-con 3.8
    24h MRP8-14 10µg n 4h
    human blood monocyte
    GSM1505870
    LPS-LPS 1.13
    24h LPS 25pg LPS 1ng 4h
    human blood monocyte
    GSM1505871
    LPS-LPS 2.13
    24h LPS 25pg LPS 1ng 4h
    human blood monocyte
    GSM1505872
    LPS-LPS 3.13
    24h LPS 25pg LPS 1ng 4h
    human blood monocyte
    GSM1505873
    MRP8/14-LPS 1.14
    24h MRP8-14 10µg LPS 1ng 4h
    human blood monocyte
    GSM1505874
    MRP8/14-LPS 2.14
    24h MRP8-14 10µg LPS 1ng 4h
    human blood monocyte
    GSM1505875
    MRP8/14-LPS 3.14
    24h MRP8-14 10µg LPS 1ng 4h
    human blood monocyte

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