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SIDRA

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Description

Human monocyte activation with NOD2L vs. TLR2/1L

Purpose

In this study, we identified genes that were differentially expressed in human monocytes activated with eiter NOD2L and/or TLR2/1L.

Experimental Design

Human blood monocytes were purified from healthy donors by Ficoll, Percoll and adherence. Monocytes were activated using NOD2L (MDP) and the TLR2/1L (19kD, triacylated peptide). Cells were harvested before activation (0h) and 6h and 24h after stimulation with ligands.

Additional Information
  • Schenk M, Krutzik SR, Sieling PA, Lee DJ et al. NOD2 triggers an interleukin-32-dependent human dendritic cell program in leprosy. Nat Med 2012 Mar 25;18(4):555-63. PMID: 22447076
  • Platform Affymetrix HG-U133_Plus_2
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