Expression data from intermediate monocytes from healthy donors and autoimmune uveitis patients-GSE66936

To compare the gene expression profiling 1) on intermediate monocytes CD14++CD16+ monocytes between healthy donors and autoimmune uveitis patients and 2) among 3 monocyte subsets in health donors

Pheripharal blood from 5 autoimmune uveitis patients and 4 healthy donors were collected. Immediately after collection, we purified circulating intermediate CD14++CD16+ monocytes from 5 patients with autoimmune uveitis ( Sample 13-17; titled as P intermediate 1-5) and 4 healthy donors (Sample 1, 4, 7, 10; titled as HD intermediate 1-4) by flow cytometry and isolated total RNA to proceed microarray assay. In addition, we also purified CD14+CD16++ (Sample 3, 6, 9, 12; titled HD non-classical 1-4) and CD14++CD16- (Sample 2, 5, 8, 11; titled HD classical 1-4) from 4 healthy donors to do microarray.

Please note that data processing was performed in two groups;[1] HD intermediate, classical and non-classical samples (total 12 samples)[2] HD intermediate samples reanalyzed with P intermediate samples (total 9 samples).