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SIDRA

GXB

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Description

Microarray analysis of IL-10 stimulated adherent peripheral blood mononuclear cells-GSE43700

Experimental Design

Peripheral blood monuclear cells derived through Ficoll-Hypaque from the blood of healthy human donors (n=4). Cells adhered to tissue culture treated 6-well plates for 2 hours in 1% Fetal Bovine Serum (FBS) RPMI and then stimulated by IL-10 (R&D Systems) 10ng/ml or media alone in 10% FBS RPMI for 24 hours at 37°C, 5% CO2. After stimulation, cells harvested and monocytes isolated through CD14 positive selection (Miltenyi Biotec). RNA from purified monocytes extracted by Trizol and purified by Qiagen RNeasy Kit. RNA probe and microarray performed by UCLA Clinical Microarray Core using Ambion labeling kit and Affymetrix Human U133 Plus 2.0 array.

Additional Information
  • Teles RM, Graeber TG, Krutzik SR, Montoya D et al. Type I interferon suppresses type II interferon-triggered human anti-mycobacterial responses.Science 2013 Mar 22;339(6126):1448-53. PMID: 23449998
  • Platform Affymetrix HG-U133_Plus_2
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