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SIDRA

GXB

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Description GSE8921_family
Experimental Design

The monocyte microarrays were generated using primary human monocytes stimulated with a TLR2/1L or media at 0, 3, 6, 12, 24 hour timepoints. A total of 10 donors were used with time 0 h samples prepared for each. For time points, we used cells from 4 of the 10 donors to prepare media and TLR2/1-stimulated samples.Dendritic cells were generated by culturing primary human monocytes with GM-CSF (800 U/ml) and IL-4 (1000 U/ml) for 7 days. The cells were then harvested and recultured for 12 hours with TLR2/1L or media. A total of four donors were used.

Additional Information
  • Liu PT, Stenger S, Li H, Wenzel L et al. Toll-like receptor triggering of a vitamin D-mediated human antimicrobial response. Science 2006 Mar 24;311(5768):1770-3. PMID: 16497887
  • Platform Affymetrix HG-U133A
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