Generation 1 is described in the 2008 Immunity paper, "A Modular Analysis Framework for Blood Genomics Studies: Application to Systemic Lupus Erythematosus". This generation has 4,742 probes assigned to 28 modules. The cell type was PBMCs and the platform was Affymetrix HG-U133A & U133B. There were 8 biological conditions/diseases used to create generation 1: arthritis, lupus, T1D, melanoma, 3 acute infections and liver transplant.
Generation 2 has 14,424 probes assigned to 260 modules. The cell type was whole blood and the platform was Illumina HT-12 V2. There were 9 biological conditions/diseases used to create generation 2.
| Perturbation | Disease | # cases | # controls |
|---|---|---|---|
| Infectious | Melioidosis/Sepsis | 9 | 24 |
| Melioidosis/Sepsis | 9 | 19 | |
| HIV | 36 | 46 | |
| TB | 6 | 9 | |
| Autoimmune | SLE | 14 | 80 |
| SLE | 12 | 15 | |
| SoJIA | 21 | 65 | |
| Immune Deficiency | B-cell deficiency | 9 | 10 |
| Tolerance | Liver Transplant | 16 | 10 |
Generation 3 has 14,504 probes assigned to 382 modules. The cell type was whole blood and the platform was Illumina HT-12 V3. There were 16 biological conditions/diseases used to create generation 3: TB, staph, sepsis, HIV, Flu, RSV, B-cell deficiency, liver transplant, pregnancy, melanoma stage IV, Kawasaki, juvenile dermatomyositis, COPD, MS untreated, Pediatric SLE, and SoJIA.